GNAQ polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GNAQ.
Immunogen
A synthetic peptide corresponding to amino acids 162-175 at internal region of human GNAQ.
Sequence
C-NDLDRVADPAYLPT
Host
Goat
Theoretical MW (kDa)
42.1
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GNAQ polyclonal antibody (Cat # PAB19720) (0.1 ug/mL) staining of rat testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GNAQ
Entrez GeneID
2776Protein Accession#
NP_002063.2Gene Name
GNAQ
Gene Alias
G-ALPHA-q, GAQ
Gene Description
guanine nucleotide binding protein (G protein), q polypeptide
Omim ID
600998Gene Ontology
HyperlinkGene Summary
Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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