COPS3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of COPS3.
Immunogen
A synthetic peptide corresponding to internal region of human COPS3.
Sequence
C-SLYKKNIQRLT
Host
Goat
Theoretical MW (kDa)
50
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:4000)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human COPS3 with DYKDDDDK tag probed with COPS3 polyclonal antibody (Cat # PAB19717) (1.0 ug/mL) in lane 1 and probed with anti-DYKDDDDK Tag (1/5000) in lane 3. Mock-transfected HEK293 probed with COPS3 polyclonal antibody (Cat # PAB19717) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — COPS3
Entrez GeneID
8533Protein Accession#
NP_003644.2Gene Name
COPS3
Gene Alias
CSN3, SGN3
Gene Description
COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
Omim ID
604665Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq
Other Designations
COP9 complex subunit 3|COP9 constitutive photomorphogenic homolog subunit 3|JAB1-containing signalosome subunit 3
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