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Last updated: 2017/2/26

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POU2AF1 polyclonal antibody

  • Catalog # : PAB19692
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of POU2AF1.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human POU2AF1.
  • Sequence:
  • C-DSDAYALNHTLSVE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 38
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (1-2 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human POU2AF1 with DYKDDDDK tag probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • POU2AF1 polyclonal antibody (Cat # PAB19692) (3-5 ug/mL) staining of paraffin embedded human spleen, shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5450
  • Gene Name:
  • POU2AF1
  • Gene Alias:
  • BOB1,OBF-1,OBF1,OCAB
  • Gene Description:
  • POU class 2 associating factor 1
  • Other Designations:
  • POU domain, class 2, associating factor 1
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