POU2AF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human POU2AF1 with DYKDDDDK tag probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
POU2AF1 polyclonal antibody (Cat # PAB19692) (3-5 ug/mL) staining of paraffin embedded human spleen, shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU2AF1.
Immunogen
A synthetic peptide corresponding to C-terminus of human POU2AF1.
Sequence
C-DSDAYALNHTLSVE
Host
Goat
Theoretical MW (kDa)
38
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human POU2AF1 with DYKDDDDK tag probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with POU2AF1 polyclonal antibody (Cat # PAB19692) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
POU2AF1 polyclonal antibody (Cat # PAB19692) (3-5 ug/mL) staining of paraffin embedded human spleen, shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU2AF1
Entrez GeneID
5450Protein Accession#
NP_006226.2Gene Name
POU2AF1
Gene Alias
BOB1, OBF-1, OBF1, OCAB
Gene Description
POU class 2 associating factor 1
Omim ID
601206Gene Ontology
HyperlinkGene Summary
class 2
Other Designations
POU domain, class 2, associating factor 1
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Interactome
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Disease
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