CHRNA5 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CHRNA5.
Immunogen
A synthetic peptide corresponding to internal region of human CHRNA5.
Sequence
C-HVDRYFTQKEET
Host
Goat
Theoretical MW (kDa)
53.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CHRNA5 polyclonal antibody (Cat # PAB19688) (1 ug/mL) staining of KELLY lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHRNA5
Entrez GeneID
1138Protein Accession#
NP_000736.2Gene Name
CHRNA5
Gene Alias
LNCR2
Gene Description
cholinergic receptor, nicotinic, alpha 5
Omim ID
118505Gene Ontology
HyperlinkGene Summary
Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. See MIM 118508 for additional background information on AChRs.[supplied by OMIM
Other Designations
Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5|cholinergic receptor, nicotinic, alpha polypeptide 5|neuronal nicotinic acetylcholine receptor, alpha5 subunit
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Interactome
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Disease
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Publication Reference
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Multiple isoforms and differential allelic expression of CHRNA5 in lung tissue and lung adenocarcinoma.
Falvella FS, Alberio T, Noci S, Santambrogio L, Nosotti M, Incarbone M, Pastorino U, Fasano M, Dragani TA.
Carcinogenesis 2013 Jun; 34(6):1281.
Application:WB, Human, A549 cells.
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Multiple isoforms and differential allelic expression of CHRNA5 in lung tissue and lung adenocarcinoma.
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