ANGPT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human ANGPT1 with DYKDDDDK tag probed with ANGPT1 polyclonal antibody (Cat # PAB19687) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with ANGPT1 polyclonal antibody (Cat # PAB19687) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ANGPT1.
Immunogen
A synthetic peptide corresponding to internal region of human ANGPT1.
Sequence
C-PDFSSQKLQH
Host
Goat
Theoretical MW (kDa)
48
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human ANGPT1 with DYKDDDDK tag probed with ANGPT1 polyclonal antibody (Cat # PAB19687) (1 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/1000) in lane 3. Mock-transfected HEK293 probed with ANGPT1 polyclonal antibody (Cat # PAB19687) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ANGPT1
Entrez GeneID
284Protein Accession#
NP_001137.2Gene Name
ANGPT1
Gene Alias
AGP1, AGPT, ANG1
Gene Description
angiopoietin 1
Omim ID
601667Gene Ontology
HyperlinkGene Summary
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. [provided by RefSeq
Other Designations
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Interactome
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Disease
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