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Last updated: 2022/5/22

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NCAM1 (isoform 2) polyclonal antibody 

  • Catalog # : PAB19678
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NCAM1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human NCAM1.
  • Sequence:
  • C-KASWTRPEKQETLD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 94.6
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:32000)
    Flow Cytometry (10 ug/mL)
    Immunohistochemistry (5 ug/mL)
    Western Blot (0.01-0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) (0.01 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) (5 ug/mL) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 4684
  • Gene Name:
  • NCAM1
  • Gene Alias:
  • CD56,MSK39,NCAM
  • Gene Description:
  • neural cell adhesion molecule 1
  • Other Designations:
  • antigen recognized by monoclonal antibody 5.1H11,neural cell adhesion molecule, NCAM
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