NCAM1 (isoform 2) polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NCAM1.
Immunogen
A synthetic peptide corresponding to internal region of human NCAM1.
Sequence
C-KASWTRPEKQETLD
Host
Goat
Theoretical MW (kDa)
94.6
Reactivity
Human
Specificity
This antibody is expected to recognize reported isoform 2 (NP_851996.2) only.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (5 ug/mL)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) (0.01 ug/mL) staining of human frontal cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) (5 ug/mL) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
NCAM1 (isoform 2) polyclonal antibody (Cat # PAB19678) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — NCAM1
Entrez GeneID
4684Protein Accession#
NP_851996.2Gene Name
NCAM1
Gene Alias
CD56, MSK39, NCAM
Gene Description
neural cell adhesion molecule 1
Omim ID
116930Gene Ontology
HyperlinkGene Summary
NCAM
Other Designations
antigen recognized by monoclonal antibody 5.1H11|neural cell adhesion molecule, NCAM
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Interactome
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Pathway
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Disease
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