SOD2 (isoform A) polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of SOD2.
Immunogen
A synthetic peptide corresponding to internal region of human SOD2.
Sequence
C-DVTAQIALQPALKFN
Host
Goat
Theoretical MW (kDa)
24.8
Reactivity
Human
Specificity
This antibody is expected to recognize isoform A (NP_000627.2) only. Reported variants represent identical protein: NP_000627.2; NP_001019636.1.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SOD2 (isoform A) polyclonal antibody (Cat # PAB19675) (0.01 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOD2
Entrez GeneID
6648Protein Accession#
NP_000627.2Gene Name
SOD2
Gene Alias
IPO-B, MNSOD, Mn-SOD
Gene Description
superoxide dismutase 2, mitochondrial
Omim ID
147460Gene Ontology
HyperlinkGene Summary
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
Mn superoxide dismutase|OTTHUMP00000017530|OTTHUMP00000017531|indophenoloxidase B|manganese superoxide dismutase|manganese-containing superoxide dismutase|mangano-superoxide dismutase
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