IDS polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
IDS polyclonal antibody (Cat # PAB19674) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IDS polyclonal antibody (Cat # PAB19674) (3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IDS.
Immunogen
A synthetic peptide corresponding to internal region of human IDS.
Sequence
C-KHFRFRDLEEDP
Host
Goat
Theoretical MW (kDa)
61.9
Reactivity
Human
Specificity
This antibody is expected to recognize isoform a (NP_000193.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
IDS polyclonal antibody (Cat # PAB19674) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IDS polyclonal antibody (Cat # PAB19674) (3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IDS
Entrez GeneID
3423Protein Accession#
NP_000193.1Gene Name
IDS
Gene Alias
MPS2, SIDS
Gene Description
iduronate 2-sulfatase
Omim ID
309900Gene Ontology
HyperlinkGene Summary
Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described. [provided by RefSeq
Other Designations
Hunter syndrome|OTTHUMP00000024207|OTTHUMP00000024210|alpha-L-iduronate sulfate sulfatase|iduronate-2-sulfatase|idursulfase
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Interactome
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Pathway
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Disease
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