HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human GM2A with DYKDDDDK tag probed with GM2A polyclonal antibody (Cat # PAB19671) (1.0 ug/mL) in lane 1 and probed with anti- DYKDDDDK Tag (1/30000) in lane 3. Mock-transfected HEK293 probed with GM2A polyclonal antibody (Cat # PAB19671) (1 mg/mL) in lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
This gene encodes a small glycolipid transport protein which acts as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. Beta-hexosaminidase A, together with GM2 ganglioside activator, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease. [provided by RefSeq
cerebroside sulfate activator protein,sphingolipid activator protein 3