SDF4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SDF4.
Immunogen
A synthetic peptide corresponding to amino acids 161-175 at internal region of human SDF4.
Sequence
C-EYKVKFLASKGHSEK
Host
Goat
Theoretical MW (kDa)
41.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:64000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (5-10 ug/mL)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SDF4 polyclonal antibody (Cat # PAB19667) (0.1 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SDF4 polyclonal antibody (Cat # PAB19667) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
SDF4 polyclonal antibody (Cat # PAB19667) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
SDF4 polyclonal antibody (Cat # PAB19667) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — SDF4
Entrez GeneID
51150Protein Accession#
NP_057631.1;NP_057260.2Gene Name
SDF4
Gene Alias
Cab45
Gene Description
stromal cell derived factor 4
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000001752|OTTHUMP00000001755|calcium binding protein
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Interactome
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Disease
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