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Last updated: 2022/7/3

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SDF4 polyclonal antibody 

  • Catalog # : PAB19667
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SDF4.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 161-175 at internal region of human SDF4.
  • Sequence:
  • C-EYKVKFLASKGHSEK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 41.8
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:64000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (5-10 ug/mL)
    Western Blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SDF4 polyclonal antibody (Cat # PAB19667) (0.1 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • SDF4 polyclonal antibody (Cat # PAB19667) (5 ug/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • SDF4 polyclonal antibody (Cat # PAB19667) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • SDF4 polyclonal antibody (Cat # PAB19667) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Protein Accession#:
  • NP_057631.1;NP_057260.2
  • Gene Name:
  • SDF4
  • Gene Alias:
  • Cab45
  • Gene Description:
  • stromal cell derived factor 4
  • Other Designations:
  • OTTHUMP00000001752,OTTHUMP00000001755,calcium binding protein
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