ZEB2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ZEB2.
Immunogen
A synthetic peptide corresponding to amino acids 545-558 at internal region of human ZEB2.
Sequence
C-TDSRRQISNIKKEK
Host
Goat
Theoretical MW (kDa)
136
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_055610.1; NP_001165124.1).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ZEB2 polyclonal antibody (Cat # PAB19665) (0.2 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZEB2
Entrez GeneID
9839Protein Accession#
NP_055610.1;NP_001165124.1Gene Name
ZEB2
Gene Alias
KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B
Gene Description
zinc finger E-box binding homeobox 2
Gene Ontology
HyperlinkGene Summary
The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM
Other Designations
Smad interacting-protein 1|Smad-interacting protein 1|zinc finger E-box-binding protein 2|zinc finger homeobox 1b
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Interactome
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Disease
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