DIAPH1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DIAPH1.
Immunogen
A synthetic peptide corresponding to internal region of human DIAPH1.
Sequence
C-QTKTSKAKKDQ
Host
Goat
Theoretical MW (kDa)
141, 139
Reactivity
Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_005210.3; NP_001073280.1).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DIAPH1 polyclonal antibody (Cat # PAB19662) (0.3 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DIAPH1
Entrez GeneID
1729Protein Accession#
NP_005210.3;NP_001073280.1Gene Name
DIAPH1
Gene Alias
DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1
Gene Description
diaphanous homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000195047|OTTHUMP00000195048|diaphanous 1|diaphanous-1|diaphanous-related formin 1
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Interactome
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Pathway
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Disease
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