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Last updated: 2022/5/22

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RORC polyclonal antibody 

  • Catalog # : PAB19661
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of RORC.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 200-212 at internal region of human RORC.
  • Sequence:
  • CHLEYSPERGKAE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 55.8
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_005051.2; NP_001001523.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • ELISA (1:16000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.01-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • RORC polyclonal antibody (Cat # PAB19661) (0.1 ug/mL) staining of Human Colon (A), (0.03 ug/mL) Liver (B) and (0.01 ug/mL) Testes (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • RORC polyclonal antibody (Cat # PAB19661) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear, cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • RORC polyclonal antibody (Cat # PAB19661) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 6097
  • Protein Accession#:
  • NP_005051.2;NP_001001523.1
  • Gene Name:
  • RORC
  • Gene Alias:
  • MGC129539,NR1F3,RORG,RZR-GAMMA,RZRG,TOR
  • Gene Description:
  • RAR-related orphan receptor C
  • Gene Summary:
  • The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000015253,RAR-related orphan receptor C, isoform a,RAR-related orphan receptor gamma,nuclear receptor ROR-gamma,retinoic acid-binding receptor gamma,retinoid-related orphan receptor gamma
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