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IDH3G polyclonal antibody

  • Catalog # : PAB19660
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of IDH3G.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 337-350 at internal region of human IDH3G.
  • Sequence:
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 41.5
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Recommend Usage:
  • Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • IDH3G polyclonal antibody (Cat # PAB19660) (0.5 ug/mL) staining of MCF-7 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3421
  • Protein Accession#:
  • NP_004126.1;NP_777358.1
  • Gene Name:
  • IDH3G
  • Gene Alias:
  • H-IDHG
  • Gene Description:
  • isocitrate dehydrogenase 3 (NAD+) gamma
  • Gene Summary:
  • Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq
  • Other Designations:
  • IDH-gamma,NAD (H)-specific isocitrate dehydrogenase gamma subunit,NAD+-specific ICDH,OTTHUMP00000025985,isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit,isocitric dehydrogenase
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