ACPP polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ACPP polyclonal antibody (Cat # PAB19651) (0.3 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACPP polyclonal antibody (Cat # PAB19651) (3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACPP.
Immunogen
A synthetic peptide corresponding to internal region of human ACPP.
Sequence
C-YGIHKQKEKSRLQ
Host
Goat
Theoretical MW (kDa)
44.6
Reactivity
Human
Specificity
This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACPP polyclonal antibody (Cat # PAB19651) (0.3 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACPP polyclonal antibody (Cat # PAB19651) (3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACPP
Entrez GeneID
55Protein Accession#
NP_001090.2Gene Name
ACPP
Gene Alias
ACP-3, ACP3, PAP
Gene Description
acid phosphatase, prostate
Omim ID
171790Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq
Other Designations
prostatic acid phosphotase
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Interactome
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Pathway
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Disease
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