ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used to detect phospho-ERBB2 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ug/mL) for 15 min ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Dot Blot (Peptide)
Dot blot analysis of ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq