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Last updated: 2017/8/20
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ERBB2 (phospho Y1248) polyclonal antibody

  • Catalog # : PAB1958
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1248 of human ERBB2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:8000)
    Immunohistochemistry (1:50-1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used to detect phospho-ERBB2 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ug/mL) for 15 min ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2064
  • Protein Accession#:
  • NP_004439;P04626
  • Gene Name:
  • ERBB2
  • Gene Alias:
  • CD340,HER-2,HER-2/neu,HER2,NEU,NGL,TKR1
  • Gene Description:
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • Gene Summary:
  • This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
  • Other Designations:
  • c-erb B2/neu protein,erbB-2,herstatin,neuroblastoma/glioblastoma derived oncogene homolog,v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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