ZIC3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of ZIC3 polyclonal antibody (Cat # PAB1954) in HL-60 cell line lysates (35 ug/lane).ZIC3 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ZIC3 polyclonal antibody (Cat # PAB1954), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ZIC3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ZIC3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ZIC3 polyclonal antibody (Cat # PAB1954) in HL-60 cell line lysates (35 ug/lane).ZIC3 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ZIC3 polyclonal antibody (Cat # PAB1954), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — ZIC3
Entrez GeneID
7547Protein Accession#
NP_003404;O60481Gene Name
ZIC3
Gene Alias
HTX, HTX1, ZNF203
Gene Description
Zic family member 3 (odd-paired homolog, Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq
Other Designations
OTTHUMP00000024142|heterotaxy 1|zinc finger protein of the cerebellum 3
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Interactome
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Disease
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Publication Reference
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Characterization of the interactions of human ZIC3 mutants with GLI3.
Zhu L, Zhou G, Poole S, Belmont JW.
Human Mutation 2008 Jan; 29(1):99.
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Identification of a novel role of ZIC3 in regulating cardiac development.
Zhu L, Harutyunyan KG, Peng JL, Wang J, Schwartz RJ, Belmont JW.
Human Molecular Genetics 2007 Jul; 16(14):1649.
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Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3.
Bedard JE, Purnell JD, Ware SM.
Human Molecular Genetics 2007 Jan; 16(2):187.
Application：IF, Human, Mouse, HeLa, NIH/3T3 cells.
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Characterization of the interactions of human ZIC3 mutants with GLI3.
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