PF4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PF4.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human PF4.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinate human PF4 protein with PF4 polyclonal antibody (Cat # PAB19529).
Lane 1, 10 ng.
Lane 2, 5 ng.
Lane 3, 2.5 ng.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — PF4
Entrez GeneID
5196Gene Name
PF4
Gene Alias
CXCL4, MGC138298, SCYB4
Gene Description
platelet factor 4
Omim ID
173460Gene Ontology
HyperlinkGene Summary
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM
Other Designations
chemokine (C-X-C motif) ligand 4|platelet factor 4 (chemokine (C-X-C motif) ligand 4)
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