Western blot analysis of tissue and cell extracts with PIK3R1 polyclonal antibody (Cat # PAB19524). Lane 1, rat skeletal muscle tissue lysate. Lane 2, rat liver tissue lysate. Lane 3, rat brain tissue lysate. Lane 4, rat small intestine tissue lysate. Lane 5, MCF-7 whole cell lysate. Lane 6, HeLa whole cell lysate. Lane 7, SMMC whole cell lysate. Lane 8, Jurkat whole cell lysate. Lane 9, COLO 320 whole cell lysate.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq