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SPP1 polyclonal antibody

  • Catalog # : PAB19513
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SPP1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at N-terminus of human SPP1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • It different from the mouse sequence by six amino acids.
  • Form:
  • Lyophilized
  • Purification:
  • Immunoaffinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of whole cell extracts with SPP1 polyclonal antibody (Cat # PAB19513).
    Lane 1, mm231
    Lane 2, HeLa
    Lane 3, Jurkat
    Lane 4, COLO 320
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human breast cancer tissue section with SPP1 polyclonal antibody (Cat # PAB19513).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6696
  • Gene Name:
  • SPP1
  • Gene Alias:
  • BNSP,BSPI,ETA-1,MGC110940,OPN
  • Gene Description:
  • secreted phosphoprotein 1
  • Other Designations:
  • SPP1/CALPHA1 fusion,bone sialoprotein I,early T-lymphocyte activation 1,osteopontin,osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein,secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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