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Last updated: 2017/11/19

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TRMT12 polyclonal antibody

  • Catalog # : PAB19449
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TRMT12.
  • Immunogen:
  • A synthetic peptide corresponding to 19 amino acids near N-terminus of human TRMT12.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • TRMT12 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms.
  • Form:
  • Liquid
  • Purification:
  • Peptide affinity purification
  • Concentration:
  • 1 mg/mL
  • Recommend Usage:
  • Western Blot (0.5 ug/mL)
    Immunocytochemistry (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of TYW2 in K-562 cell lysate with TRMT12 polyclonal antibody (Cat # PAB19449) at 0.5 ug/mL.
    Lane 1 : The absence of blocking peptide.
    Lane 2 : The presence of blocking peptide.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of K-562 cells with TRMT12 polyclonal antibody (Cat # PAB19449) at 10 ug/mL.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • TRMT12
  • Gene Alias:
  • FLJ20772,TRM12,TYW2
  • Gene Description:
  • tRNA methyltransferase 12 homolog (S. cerevisiae)
  • Gene Summary:
  • Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
  • Other Designations:
  • homolog of yeast tRNA methyltransferase,homolog of yeast tRNA methyltransferase 12,tRNA methyltranferase 12 homolog,tRNA-yW synthesizing protein 2
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