LCMT2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of TYW4 in rat brain tissue lysate with LCMT2 polyclonal antibody (Cat # PAB19442) at 1 ug/mL.
Lane 1 : The absence of blocking peptide.
Lane 2 : The presence of blocking peptide.
Immunohistochemistry
Immunohistochemical staining of mouse brain cells with LCMT2 polyclonal antibody (Cat # PAB19442) at 5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LCMT2.
Immunogen
A synthetic peptide corresponding to 16 amino acids near N-terminus of human LCMT2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of TYW4 in rat brain tissue lysate with LCMT2 polyclonal antibody (Cat # PAB19442) at 1 ug/mL.
Lane 1 : The absence of blocking peptide.
Lane 2 : The presence of blocking peptide.Immunohistochemistry
Immunohistochemical staining of mouse brain cells with LCMT2 polyclonal antibody (Cat # PAB19442) at 5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LCMT2
Entrez GeneID
9836Protein Accession#
NP_055608Gene Name
LCMT2
Gene Alias
KIAA0547, MGC9534, PPM2, TYW4
Gene Description
leucine carboxyl methyltransferase 2
Omim ID
611246Gene Ontology
HyperlinkGene Summary
The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq
Other Designations
OTTHUMP00000161298|tRNA-Wybutosine-synthesizing protein 4|tRNA-yW synthesizing protein 4
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Interactome
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Pathway
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