ROCK1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 polyclonal antibody (Cat # PAB19432) at 1 ug/mL.
Lane 1 : The absence of blocking peptide.
Lane 2 : The presence of blocking peptide.
Immunocytochemistry
Immunocytochemical staining of 293 cells with ROCK1 polyclonal antibody (Cat # PAB19432) at 10 ug/mL.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROCK1.
Immunogen
A synthetic peptide corresponding to 15 amino acids near N-terminus of human ROCK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
Immunocytochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 polyclonal antibody (Cat # PAB19432) at 1 ug/mL.
Lane 1 : The absence of blocking peptide.
Lane 2 : The presence of blocking peptide.Immunocytochemistry
Immunocytochemical staining of 293 cells with ROCK1 polyclonal antibody (Cat # PAB19432) at 10 ug/mL.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — ROCK1
Entrez GeneID
6093Protein Accession#
NP_005397Gene Name
ROCK1
Gene Alias
MGC131603, MGC43611, P160ROCK, PRO0435
Gene Description
Rho-associated, coiled-coil containing protein kinase 1
Omim ID
601702Gene Ontology
HyperlinkGene Summary
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq
Other Designations
OTTHUMP00000162514|p160-ROCK
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com