SPTLC2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SPTLC2 in NIH/3T3 cell lysate with SPTLC2 polyclonal antibody (Cat # PAB19384) at 1 ug/mL in (1) the absence and (2) the presence of blocking peptide.
Immunocytochemistry
Immunocytochemical staining of 3T3 cells with SPTLC2 polyclonal antibody (Cat # PAB19384) at 10 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SPTLC2.
Immunogen
A synthetic peptide corresponding to 15 amino acids near C-terminus of human SPTLC2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least three isoforms of SPTLC2 are known to exist; this antibody will not detect isoform b. This antibody is predicted to not cross-react with SPT1.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
Immunocytochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SPTLC2 in NIH/3T3 cell lysate with SPTLC2 polyclonal antibody (Cat # PAB19384) at 1 ug/mL in (1) the absence and (2) the presence of blocking peptide.Immunocytochemistry
Immunocytochemical staining of 3T3 cells with SPTLC2 polyclonal antibody (Cat # PAB19384) at 10 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPTLC2
Entrez GeneID
9517Protein Accession#
NP_4854Gene Name
SPTLC2
Gene Alias
KIAA0526, LCB2, SPT2
Gene Description
serine palmitoyltransferase, long chain base subunit 2
Omim ID
605713Gene Ontology
HyperlinkGene Summary
This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
serine palmitoyltransferase, subunit II
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Interactome
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