RABGEF1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RABGEF1.
Immunogen
A synthetic peptide corresponding to 19 amino acids near internal region of human RABGEF1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least four isoforms of RABGEF1 are known to exist; this antibody will recognize all four. This antibody is predicted to not cross-react with other LRRFIP family members.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of RABGEF1 in rat brain tissue lysate with RABGEF1 polyclonal antibody (Cat # PAB19371) at 1 ug/mL.Immunohistochemistry
Immunohistochemical staining of mouse brain cells with RABGEF1 polyclonal antibody (Cat # PAB19371) at 5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RABGEF1
Entrez GeneID
27342Protein Accession#
NP_055319Gene Name
RABGEF1
Gene Alias
FLJ32302, RABEX5, rabex-5
Gene Description
RAB guanine nucleotide exchange factor (GEF) 1
Omim ID
609700Gene Ontology
HyperlinkGene Summary
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM
Other Designations
Rab5 GDP/GTP exchange factor
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Interactome
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Disease
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