USP21 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of USP21 polyclonal antibody (Cat # PAB1936) in T-47D cell line lysates (35 ug/lane). USP21 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with USP21 polyclonal antibody (Cat # PAB1936) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of USP21.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 406-436 from C-terminus of human USP21.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of USP21 polyclonal antibody (Cat # PAB1936) in T-47D cell line lysates (35 ug/lane). USP21 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with USP21 polyclonal antibody (Cat # PAB1936) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — USP21
Entrez GeneID
27005Protein Accession#
NP_036607;Q9UK80Gene Name
USP21
Gene Alias
MGC3394, USP16, USP23
Gene Description
ubiquitin specific peptidase 21
Omim ID
604729Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
NEDD8-specific protease|deubiquitinating enzyme 21|ubiquitin carboxyl-terminal hydrolase 21|ubiquitin specific protease 21|ubiquitin thiolesterase 21|ubiquitin-specific processing protease 21|ubiquitin-specific protease 16|ubiquitin-specific protease 21
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Interactome
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Publication Reference
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Human and mouse proteases: a comparative genomic approach.
Puente XS, Sanchez LM, Overall CM, Lopez-Otin C.
Nature Reviews. Genetics 2003 Jul; 4(7):544.
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Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins.
Gong L, Kamitani T, Millas S, Yeh ET.
The Journal of Biological Chemistry 2000 May; 275(19):14212.
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Generation and analysis of 280,000 human expressed sequence tags.
Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M.
Genome Research 1996 Sep; 6(9):807.
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Human and mouse proteases: a comparative genomic approach.
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