ZEB2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody rasied against synthetic peptide of ZEB2.
Immunogen
A synthetic peptide corresponding to 18 amino acids near C-terminus of human ZEB2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1-2 ug/mL)
Immunocytochemistry (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ZEB2 in Jurkat cell lysate with ZEB2 polyclonal antibody (Cat # PAB19269) at (1) 1 and (2) 2ug/mL.Immunocytochemistry
Immunocytochemistry of Jurkat cells with ZEB2 polyclonal antibody (Cat # PAB19269) at 20 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZEB2
Entrez GeneID
9839Protein Accession#
AAI27103Gene Name
ZEB2
Gene Alias
KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B
Gene Description
zinc finger E-box binding homeobox 2
Gene Ontology
HyperlinkGene Summary
The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM
Other Designations
Smad interacting-protein 1|Smad-interacting protein 1|zinc finger E-box-binding protein 2|zinc finger homeobox 1b
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Interactome
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Disease
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Publication Reference
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Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Bambang IF, Lee YK, Richardson DR, Zhang D.
Oncogene 2013 Mar; 32(10):1240.
Application:WB-Tr, Human, MDA-MB-231, BT549 cells.
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Endosomal sorting by Semaphorin 4A in retinal pigment epithelium supports photoreceptor survival.
Toyofuku T, Nojima S, Ishikawa T, Takamatsu H, Tsujimura T, Uemura A, Matsuda J, Seki T, Kumanogoh A.
Genes & Development 2012 Apr; 26(8):816.
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Hepatocyte growth factor activates Wnt pathway by transcriptional activation of LEF1 to facilitate tumor invasion.
Huang FI, Chen YL, Chang CN, Yuan RH, Jeng YM.
Carcinogenesis 2012 Jun; 33(6):1142.
Application:WB-Tr, Human, MDA-MB-231 cells.
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Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
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