SNAI1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of NCI-H460 cell lysate (35 ug/lane) with SNAI1 polyclonal antibody (Cat # PAB1925).
Western Blot (Transfected lysate)
Western blot analysis of SNAI1 (arrow) using SNAI1 polyclonal antibody (Cat # PAB1925).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SNAI1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SNAI1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NCI-H460 cell lysate (35 ug/lane) with SNAI1 polyclonal antibody (Cat # PAB1925).Western Blot (Transfected lysate)
Western blot analysis of SNAI1 (arrow) using SNAI1 polyclonal antibody (Cat # PAB1925).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2). -
Gene Info — SNAI1
Entrez GeneID
6615Protein Accession#
NP_005976;O95863Gene Name
SNAI1
Gene Alias
SLUGH2, SNA, SNAH, dJ710H13.1
Gene Description
snail homolog 1 (Drosophila)
Omim ID
604238Gene Ontology
HyperlinkGene Summary
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq
Other Designations
OTTHUMP00000031680|snail 1 homolog|snail 1 zinc finger protein|snail 1, zinc finger protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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The DNA sequence and comparative analysis of human chromosome 20.
Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafh
Nature 2001 Dec; 414(6866):865.
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The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells.
Batlle E, Sancho E, Franci C, Dominguez D, Monfar M, Baulida J, Garcia De Herreros A.
Nature Cell Biology 2000 Feb; 2(2):84.
Application：WB, Human, HT-29 M6, MiaPaca-2 cells.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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