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CDC42 polyclonal antibody

  • Catalog # : PAB19100
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDC42.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human CDC42.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Human, Mouse, Rat
  • Form:
  • Lyophilized
  • Purification:
  • Immunoaffinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Western Blot (0.1-0.5 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of tissue and whole cell extracts with CDC42 polyclonal antibody (Cat # PAB19100).
    Lane 1 : rat brain.
    Lane 2 : MCF-7. Lane 3 : HeLa. Lane 4 : SMMC. Lane 5 : U87.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis of CDC42 recombinant protein with CDC42 polyclonal antibody (Cat # PAB19100).
    Lane 1 : 10 ng.
    Lane 2 : 5 ng.
    Lane 3 : 2.5 ng.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Gene Information
  • Entrez GeneID:
  • 998
  • Gene Name:
  • CDC42
  • Gene Alias:
  • CDC42Hs,G25K
  • Gene Description:
  • cell division cycle 42 (GTP binding protein, 25kDa)
  • Gene Summary:
  • The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • GTP-binding protein, 25kD,OTTHUMP00000002834,OTTHUMP00000002926,cell division cycle 42,cell division cycle 42 (GTP binding protein, 25kD),cell division cycle 42 (GTP-binding protein, 25kD),dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)),d
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