MMP9 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of tissue and whole cell extracts with MMP9 polyclonal antibody (Cat # PAB19095).
Lane 1 : rat embryo.
Lane 2 : MM453. Lane 3 : HeLa. Lane 4 : SMMC. Lane 5 : Jurkat. Lane 6 : HT1080.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer tissue with MMP9 polyclonal antibody (Cat # PAB19095).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP9.
Immunogen
A synthetic peptide corresponding to C-terminus of human MMP9.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Purification
Immunoaffinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of tissue and whole cell extracts with MMP9 polyclonal antibody (Cat # PAB19095).
Lane 1 : rat embryo.
Lane 2 : MM453. Lane 3 : HeLa. Lane 4 : SMMC. Lane 5 : Jurkat. Lane 6 : HT1080.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lung cancer tissue with MMP9 polyclonal antibody (Cat # PAB19095). -
Gene Info — MMP9
Entrez GeneID
4318Gene Name
MMP9
Gene Alias
CLG4B, GELB, MMP-9
Gene Description
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Omim ID
120361Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq
Other Designations
92kD type IV collagenase|OTTHUMP00000031674|gelatinase B|macrophage gelatinase|matrix metalloproteinase 9|matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kD
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Interactome
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Pathway
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Disease
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Publication Reference
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
Yuan CL, Liang R, Liu ZH, Li YQ, Luo XL, Ye JZ, Lin Y.
Experimental and Therapeutic Medicine 2018 Jun; 15(6):5422.
Application：WB-Tr, Human, BGC‑823 cells.
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The in vitro and in vivo effects of microRNA-133a on intervertebral disc destruction by targeting MMP9 in spinal tuberculosis.
Wang XW, Liu JJ, Wu QN, Wu SF, Hao DJ.
Life Sciences 2017 Jul; 188:198.
Application：WB-Tr, Rabbit, Rabbit primary notochordal cells.
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iTRAQ-Based Proteomics Screen identifies LIPOCALIN-2 (LCN-2) as a potential biomarker for colonic lateral-spreading tumors.
Wang X, Li A, Guo Y, Wang Y, Zhao X, Xiang L, Han Z, Li Y, Xu W, Zhuang K, Yan Q, Zhong J, Xiong J, Liu S.
Scientific Reports 2016 Jun; 6:28600.
Application：IHC, WB-Ti, Human, Colorectal.
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
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