PSMB3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PSMB3 polyclonal antibody (Cat # PAB19047) (1 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PSMB3.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human PSMB3.
Sequence
C-NLYELKEGRQ
Host
Goat
Theoretical MW (kDa)
24
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PSMB3 polyclonal antibody (Cat # PAB19047) (1 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PSMB3
Entrez GeneID
5691Protein Accession#
NP_002786.2Gene Name
PSMB3
Gene Alias
HC10-II, MGC4147
Gene Description
proteasome (prosome, macropain) subunit, beta type, 3
Omim ID
602176Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12. [provided by RefSeq
Other Designations
proteasome beta 3 subunit|proteasome chain 13|proteasome component C10-II|proteasome theta chain
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Interactome
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Pathway
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