POR polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POR.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human POR.
Sequence
C-TNPPRTNVLYE
Host
Goat
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POR polyclonal antibody (Cat # PAB19039) (0.1 ug/mL) staining of rat liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POR
Entrez GeneID
5447Protein Accession#
NP_000932.3Gene Name
POR
Gene Alias
CPR, CYPOR, DKFZp686G04235, FLJ26468, P450R
Gene Description
P450 (cytochrome) oxidoreductase
Gene Ontology
HyperlinkGene Summary
This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq
Other Designations
NADPH-dependent cytochrome P450 reductase|cytochrome P450 reductase
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