TPPP polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TPPP.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human TPPP.
Sequence
C-DPSKDRAAKRLSLES
Host
Goat
Theoretical MW (kDa)
26
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:32000)
Western Blot (0.001-0.01 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TPPP polyclonal antibody (Cat # PAB19023) (0.001 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with TPPP polyclonal antibody (Cat # PAB19023) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TPPP
Entrez GeneID
11076Protein Accession#
NP_008961.1Gene Name
TPPP
Gene Alias
TPPP/p25, TPPP1, p24, p25, p25alpha
Gene Description
tubulin polymerization promoting protein
Omim ID
608773Gene Ontology
HyperlinkOther Designations
brain specific protein p25 alpha|glycogen synthase kinase 3 (GSK3) inhibitor p24|tubulin polymerization-promoting protein
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