HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human STAT4 with C-terminal MYC tag probed with STAT4 polyclonal antibody (Cat # PAB19017) (1 ug/mL) in Lane 1 and probed with anti-MYC Tag (1/1000) in Lane 3. Mock-transfected HEK293 probed with STAT4 polyclonal antibody (Cat # PAB19017) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq