MYO5A polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MYO5A.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human MYO5A.
Sequence
C-ETKQLELDLN
Host
Goat
Theoretical MW (kDa)
200
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
Immunofluorescence (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MYO5A polyclonal antibody (Cat # PAB19013) (1 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunofluorescence
MYO5A polyclonal antibody (Cat # PAB19013) (1 ug/mL) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, Germany.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYO5A
Entrez GeneID
4644Protein Accession#
NP_000250.3;NP_001135967.1Gene Name
MYO5A
Gene Alias
GS1, MYH12, MYO5, MYR12
Gene Description
myosin VA (heavy chain 12, myoxin)
Gene Ontology
HyperlinkGene Summary
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000174452|dilute|myosin V|myosin VA|myosin heavy chain 12|myosin, heavy polypeptide kinase|myoxin
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Interactome
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Disease
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