POU4F3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU4F3.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human POU4F3.
Sequence
C-EAAYREKNSKPE
Host
Goat
Theoretical MW (kDa)
40
Reactivity
Human, Mouse
Specificity
This antibody is expected not to cross-react to the similar POU4F1 and POU4F2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.2-0.6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POU4F3 polyclonal antibody (Cat # PAB19005, 0.2 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU4F3
Entrez GeneID
5459Protein Accession#
NP_002691.1Gene Name
POU4F3
Gene Alias
BRN3C, DFNA15, MGC138412
Gene Description
POU class 4 homeobox 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq
Other Designations
POU domain, class 4, transcription factor 3
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