ILK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ILK polyclonal antibody (Cat # PAB18999, 0.5 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ILK.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human ILK.
Sequence
C-DMIVPILEKMQDK
Host
Goat
Theoretical MW (kDa)
52
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ILK polyclonal antibody (Cat # PAB18999, 0.5 ug/mL) staining of NIH/3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ILK
Entrez GeneID
3611Protein Accession#
NP_004508.1;NP_001014794.1;NP_001014795.1Gene Name
ILK
Gene Alias
DKFZp686F1765, P59
Gene Description
integrin-linked kinase
Omim ID
602366Gene Ontology
HyperlinkGene Summary
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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