EIF4E2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of EIF4E2 polyclonal antibody (Cat # PAB1899) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4E2 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with EIF4E2 polyclonal antibody (Cat # PAB1899) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4E2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EIF4E2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of EIF4E2 polyclonal antibody (Cat # PAB1899) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4E2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with EIF4E2 polyclonal antibody (Cat # PAB1899) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EIF4E2
Entrez GeneID
9470Protein Accession#
NP_004837;O60573Gene Name
EIF4E2
Gene Alias
4E-LP, 4EHP, EIF4EL3, IF4e
Gene Description
eukaryotic translation initiation factor 4E family member 2
Omim ID
605895Gene Ontology
HyperlinkOther Designations
eIF4E-like cap-binding protein|eukaryotic translation initiation factor 4E member 2|eukaryotic translation initiation factor 4E-like 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1.
Tee AR, Tee JA, Blenis J.
FEBS Letters 2004 Apr; 564(1-2):58.
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Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.
Mao M, Fu G, Wu JS, Zhang QH, Zhou J, Kan LX, Huang QH, He KL, Gu BW, Han ZG, Shen Y, Gu J, Yu YP, Xu SH, Wang YX, Chen SJ, Chen Z.
PNAS 1998 Jul; 95(14):8175.
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Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.
Rom E, Kim HC, Gingras AC, Marcotrigiano J, Favre D, Olsen H, Burley SK, Sonenberg N.
The Journal of Biological Chemistry 1998 May; 273(21):13104.
Application：WB-Ce, WB-Re, Human, HEK 293, HeLa cells, Recombinant protein.
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Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1.
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