RUFY1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RUFY1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human RUFY1.
Sequence
C-QKNEAITSFEGKT
Host
Goat
Theoretical MW (kDa)
85
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.05-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RUFY1 polyclonal antibody (Cat # PAB18985, 0.05 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RUFY1
Entrez GeneID
80230Protein Accession#
NP_079434.3;NP_001035541.1Gene Name
RUFY1
Gene Alias
FLJ22251, RABIP4, ZFYVE12
Gene Description
RUN and FYVE domain containing 1
Omim ID
610327Gene Ontology
HyperlinkOther Designations
OTTHUMP00000161521|RUN and FYVE domain-containing 1
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Interactome
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Pathway
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