NOX5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NOX5.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NOX5.
Sequence
C-EWHPFTISSAPEQKD
Host
Goat
Theoretical MW (kDa)
90
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NOX5 polyclonal antibody (Cat # PAB18982, 0.5 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOX5
Entrez GeneID
79400Protein Accession#
NP_078781.3;NP_001171708.1;NP_001171709.1Gene Name
NOX5
Gene Alias
MGC149776, MGC149777, NOX5A, NOX5B
Gene Description
NADPH oxidase, EF-hand calcium binding domain 5
Omim ID
606572Gene Ontology
HyperlinkGene Summary
NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependent manner.[supplied by OMIM
Other Designations
NADPH oxidase, EF hand calcium-binding domain 5|NOX5 variant lacking EF hands|OTTHUMP00000164443
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Interactome
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Disease
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