HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human FANCF with C-terminal MYC tag probed with FANCF polyclonal antibody (Cat # PAB18970, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with FANCF polyclonal antibody (Cat # PAB18970, 1mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq