FANCF polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FANCF.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human FANCF.
Sequence
C-RPQEELEPGIHK
Host
Goat
Theoretical MW (kDa)
40
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human FANCF with C-terminal MYC tag probed with FANCF polyclonal antibody (Cat # PAB18970, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with FANCF polyclonal antibody (Cat # PAB18970, 1mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FANCF
Entrez GeneID
2188Protein Accession#
NP_073562.1Gene Name
FANCF
Gene Alias
FAF, MGC126856
Gene Description
Fanconi anemia, complementation group F
Omim ID
603467Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq
Other Designations
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Interactome
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Disease
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