HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human PCSK9 with C-terminal MYC tag probed with PCSK9 polyclonal antibody (Cat # PAB18965, 0.5 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with PCSK9 polyclonal antibody (Cat # PAB18965, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq
OTTHUMP00000009833,convertase subtilisin/kexin type 9 preproprotein,neural apoptosis regulated convertase 1