SEPT4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SEPT4 polyclonal antibody (Cat # PAB18964, 1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT4 polyclonal antibody (Cat #PAB18964) (3.75 ug/mL) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry
SEPT4 polyclonal antibody (Cat #PAB18964) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SEPT4.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human SEPT4.
Sequence
C-RNKLTRESGTD
Host
Goat
Theoretical MW (kDa)
50
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (3.75 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SEPT4 polyclonal antibody (Cat # PAB18964, 1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT4 polyclonal antibody (Cat #PAB18964) (3.75 ug/mL) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
SEPT4 polyclonal antibody (Cat #PAB18964) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — SEPT4
Entrez GeneID
5414Protein Accession#
NP_004565.1;NP_536341.1;NP_001185642.1Gene Name
SEPT4
Gene Alias
ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2, hucep-7
Gene Description
septin 4
Omim ID
603696Gene Ontology
HyperlinkGene Summary
This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein encoded by this gene is thought to be part of a complex involved in cytokinesis. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq
Other Designations
cell division control-related protein 2|cerebral protein 7|peanut-like 2|septin-M
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Interactome
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Disease
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