MID1IP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MID1IP1 with C-terminal MYC tag probed with MID1IP1 polyclonal antibody (Cat # PAB18960, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with MID1IP1 polyclonal antibody (Cat # PAB18960, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MID1IP1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human MID1IP1.
Sequence
C-HTVLSKLTRK
Host
Goat
Theoretical MW (kDa)
28
Reactivity
Human
Specificity
Reported variants represent identical protein: NP_001092261.1, NP_067065.1, NP_001092260.1
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MID1IP1 with C-terminal MYC tag probed with MID1IP1 polyclonal antibody (Cat # PAB18960, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with MID1IP1 polyclonal antibody (Cat # PAB18960, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MID1IP1
Entrez GeneID
58526Protein Accession#
NP_067065.1Gene Name
MID1IP1
Gene Alias
FLJ10386, G12-like, MIG12, STRAIT11499, THRSPL
Gene Description
MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish))
Gene Ontology
HyperlinkOther Designations
MID1 interacting G12-like protein|MID1 interacting protein 1 (gastrulation specific G12-like)|OTTHUMP00000025759|OTTHUMP00000025760
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Interactome
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Disease
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