RBM20 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RBM20 polyclonal antibody (Cat # PAB18957, 1 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RBM20.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human RBM20.
Sequence
C-QLDKAELDERP
Host
Goat
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RBM20 polyclonal antibody (Cat # PAB18957, 1 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescenceEnzyme-linked Immunoabsorbent Assay
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Gene Info — RBM20
Entrez GeneID
282996Protein Accession#
NP_001127835.1Gene Name
RBM20
Gene Alias
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Gene Description
RNA binding motif protein 20
Gene Ontology
HyperlinkOther Designations
RNA binding protein
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Interactome
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Disease
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Publication Reference
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RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
Ito J, Iijima M, Yoshimoto N, Niimi T, Kuroda S, Maturana AD.
FEBS Letters 2016 Jul; 590(14):2262.
Application：IP-WB, Rat, Rat ventricular cardiomyocytes.
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Identification of nuclear retention domains in the RBM20 protein.
Filippello A, Lorenzi P, Bergamo E, Romanelli MG.
FEBS Letters 2013 Sep; 587(18):2989.
Application：WB-Ce, Human, HeLa, SK-N-BE cells.
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RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
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