MGAT1 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MGAT1 with C-terminal MYC tag probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MGAT1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human MGAT1.
Sequence
C-QVEKVRTNDR
Host
Goat
Theoretical MW (kDa)
55
Reactivity
Human
Specificity
Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MGAT1 with C-terminal MYC tag probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MGAT1
Entrez GeneID
4245Protein Accession#
NP_002397.2Gene Name
MGAT1
Gene Alias
GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT
Gene Description
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Omim ID
160995Gene Ontology
HyperlinkGene Summary
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase|OTTHUMP00000161546
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Interactome
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Pathway
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Disease
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Publication Reference
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Type 2 Diabetes Biomarkers and Uses Thereof.
Eustache Paramithiotis, Marc Prentki, Rèmi Rabasa-lhoret, Pascal Croteau, Joel Lanoix, Murthy S. R. Madiraju, Érik Joly
United States Patent Application Publication 2015 Nov; [Epub].
Application：IF, WB, Human, Mouse, Rat, Islets, INS832/13, MIN6 cells.
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Type 2 Diabetes Biomarkers and Uses Thereof.
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