HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MGAT1 with C-terminal MYC tag probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 ug/mL) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with MGAT1 polyclonal antibody (Cat # PAB18956, 1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq