ETFA polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ETFA polyclonal antibody (Cat # PAB18949, 0.1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ETFA.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human ETFA.
Sequence
C-KSPDTFVRTIYAGN
Host
Goat
Theoretical MW (kDa)
30-35
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ETFA polyclonal antibody (Cat # PAB18949, 0.1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ETFA
Entrez GeneID
2108Protein Accession#
NP_000117.1;NP_001121188.1Gene Name
ETFA
Gene Alias
EMA, GA2, MADD
Gene Description
electron-transfer-flavoprotein, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
electron transfer flavoprotein alpha-subunit|electron transfer flavoprotein, alpha polypeptide|glutaric aciduria II
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Interactome
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Disease
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