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ETFA polyclonal antibody

  • Catalog # : PAB18949
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ETFA.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at internal region of human ETFA.
  • Sequence:
  • C-KSPDTFVRTIYAGN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 30-35
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is expected to recognize both isoforms (NP_000117.1; NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ETFA polyclonal antibody (Cat # PAB18949, 0.1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2108
  • Protein Accession#:
  • NP_000117.1;NP_001121188.1
  • Gene Name:
  • ETFA
  • Gene Alias:
  • EMA,GA2,MADD
  • Gene Description:
  • electron-transfer-flavoprotein, alpha polypeptide
  • Gene Summary:
  • ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • electron transfer flavoprotein alpha-subunit,electron transfer flavoprotein, alpha polypeptide,glutaric aciduria II
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