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Last updated: 2017/11/19
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DCTN3 polyclonal antibody

  • Catalog # : PAB18943
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DCTN3.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at internal region of human DCTN3.
  • Sequence:
  • C-QVALGNISSKRE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 20
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • DCTN3 polyclonal antibody (Cat # PAB18943, 0.03 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_009165.1;NP_077324.1
  • Gene Name:
  • DCTN3
  • Gene Alias:
  • DCTN-22,DCTN22,MGC111190
  • Gene Description:
  • dynactin 3 (p22)
  • Gene Summary:
  • This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000021292,OTTHUMP00000021293,OTTHUMP00000045394,dynactin 3,dynactin 3, isoform 1,dynactin light chain
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