DCTN3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DCTN3 polyclonal antibody (Cat # PAB18943) (0.1 ug/mL) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Tissue lysate)
DCTN3 polyclonal antibody (Cat # PAB18943) (0.3 ug/mL) staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DCTN3 polyclonal antibody (Cat # PAB18943) (8 ug/mL) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DCTN3.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human DCTN3.
Sequence
C-QVALGNISSKRE
Host
Goat
Theoretical MW (kDa)
20
Reactivity
Human, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunohistochemistry (6-8 ug/mL)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DCTN3 polyclonal antibody (Cat # PAB18943) (0.1 ug/mL) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Tissue lysate)
DCTN3 polyclonal antibody (Cat # PAB18943) (0.3 ug/mL) staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescenceImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DCTN3 polyclonal antibody (Cat # PAB18943) (8 ug/mL) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DCTN3
Entrez GeneID
11258Protein Accession#
NP_009165.1;NP_077324.1Gene Name
DCTN3
Gene Alias
DCTN-22, DCTN22, MGC111190
Gene Description
dynactin 3 (p22)
Omim ID
607387Gene Ontology
HyperlinkGene Summary
This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000021292|OTTHUMP00000021293|OTTHUMP00000045394|dynactin 3|dynactin 3, isoform 1|dynactin light chain
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Interactome
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