TRIM72 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM72.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human TRIM72.
Sequence
C-RPQALSTNLQ
Host
Goat
Theoretical MW (kDa)
52
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:16000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL in Tris saline, pH7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TRIM72 polyclonal antibody (Cat # PAB18940, 0.01 ug/mL) staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with TRIM72 polyclonal antibody (Cat # PAB18940) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM72
Entrez GeneID
493829Protein Accession#
NP_001008275.1Gene Name
TRIM72
Gene Alias
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Gene Description
tripartite motif-containing 72
Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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Publication Reference
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Loading-associated expression of TRIM72 and caveolin-3 in antigravitational soleus muscle in mice.
Ohno Y, Sugiura T, Ohira Y, Yoshioka T, Goto K.
Physiological Reports 2014 Dec; 2(12):e12259.
Application:WB-Ce, Mouse, Soleus muscle.
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Loading-associated expression of TRIM72 and caveolin-3 in antigravitational soleus muscle in mice.
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