VEGFA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant VEGFA.
Immunogen
Recombinant protein corresponding to amino acids 177-368 of human VEGFA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:10000-1:80000)
Western Blot (1:200-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human fetal skeletal muscle lysate with VEGFA polyclonal antibody (Cat # PAB18913) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic and membrane staining with VEGFA polyclonal antibody (Cat # PAB18913) at 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
NM_001025368Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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